Coding
PduD20-mCh

Part:BBa_K562010:Design

Designed by: Frank Sargent   Group: iGEM11_Dundee   (2011-09-17)


Salty_PduD20-mCherry


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 179
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 920
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 179
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 179
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The single natural pstI site within the mCherry gene has been removed.


Source

Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence and an unknown cloning vector encoding mCherry.

References